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Two types of endo-β-1,3-glucanases were purified from green malt and their basic characteristics were studied. Molecular weights of glucanase I and glucanase II were estimated, by electrophoresis, to be 35,000 and 28,000, respectively. Purified glucanase I and II showed the highest activity at pH 5.0∼7.0 and 5.0∼8.0, respectively. The optimal temperature of purified glucanase I and II was 40℃. Purified glucanase I and glucanase II were stable at 40℃ for 60 min and at 50℃ for 30 min. All enzymes were inactivated by AgNO₃and HgC1₂ while those were not activated by various compounds tried. Km values of glucanase I and II were 1.03 ㎎/㎖, 1.20 ㎎/㎖, respectively.
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